Field test and analysis for fungus inducing the formation of agilawood from Aquilaria sinensis / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the feasibility of utilizing three fungi YNAS04, YNAS06 and YNAS08 to induce the formation of agilawood from Aquilaria sinensis in field and laboratory, respectively. METHODS: Agarwood was obtained by inoculating the fungus in the stem of A. sinensis under natural conditions, and the main ingredients of the volatile oil from induced agilawood were analyzed by GC-MS. The authenticity of the used fungi was detected by the method of morphology and ribosomal internal transcribed spacer. RESULTS: The weights of the induced materials from YNAS04, YNAS06 and YNAS08 were (1.786±0.473), (2964±0.492) and (3.615±0.591) g after six months, which were heavier than blank control (1.325±0.407) g and negative control (1.462±0.417) g, respectively. The fungus inoculated in the stem of A. sinensis and became the dominant fungi in the tissue under natural conditions. Sesquterpenoids and chromone analogues were detected from three fungi inducing materials of six months old. CONCLUSION: Utilizing fungi to induce the formation of agilawood from Aquilaria sinensis has the advantages of spending less time and producing high-quality secondary metabolites. This method has important application foreground in large-scale agilawood production.