HPLC-ELSD determination of Fructopyrano-(l → 4)-glucopyranose in Radix Isatidis / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1404-1406, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-860637
ABSTRACT
OBJECTIVE:
To establish an HPLC-ELSD method to determine a new compound named Fructopyrano-(1 → 4)-glucopyranose in Radix Isatidis.METHODS:
The HPLC method was conducted on an Agilent XDB-C18 column (4.6 mm × 150 mm, 5 μm), with methanol-water (595) as the mobile phase. The drift tube temperature was 115°C and flow fate of carrier gas was 3 L · min-1.RESULTS:
The calibration curve was linear for FG in the range of 8.40 - 21.00 ng (r = 0.9990). The recovery was 97.27% - 102.72%.CONCLUSION:
This method is accurate, reliable, rapid, sensitive, reproducible, and can be used for the determination of FG in Radix Isatidis. Copyright 2012 by the Chinese Pharmaceutical Association.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2012
Type:
Article
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