Quantitative analysis of salviaflaside and rosmarinic acid in Prunella vulgaris / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an effective HPLC method for determinating the contents of salviaflaside and rosmarinic acid in Prunella vulgaris. METHODS: An Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) was used with gradient mobile phase of acetonitrile-water(1.0% acetic acid) at a flow rate of 1.1 mL · min-1. UV detection wavelength was 319 nm, and the column temperature was 30°C, the sample injection volume was 10 μL. RESULTS: Good linearity was obtained in the range of 0.0067-4.2 μg and 0.047-9.4 μg for salviaflaside and rosmarinic acid, respectively. The method recoveries of salviaflaside and rosmarinic acid were 99.99% and 100.2%, RSDs were 1.13% and 1.64%, respectively. The contents of salviaflaside and rosmarinic acid were more than 0.1‰ and 1%, respectively. CONCLUSION: The HPLC method is simple, rapid, accurate and suitable for the determination of salviaflaside and rosmarinic acid, which can be used for the quality control of Prunella vulgaris L. Copyright 2012 by the Chinese Pharmaceutical Association.