Simultaneous determination of two constituents in compound resorcinol cream by HPLC gradient elution / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1254-1256, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-860668
ABSTRACT
OBJECTIVE:
To establish an HPLC method with gradient elution for the determination of resorcinol and triamcinolone acetonide acetate in compound resorcinol cream.METHODS:
The HPLC was conducted on a ZORBAX Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile (A)-0.5% ammonium acetate solution (B) as the mobile phase. The gradient elution program was as follows 0-3 min (25% A), 3-5 min (25% A-55% A), and 5-15 min (55% A). The column temperature was 40°C. The flow rate was 1.0 mL · min-1 and the detection wavelength was 240 nm.RESULTS:
The linear ranges of resorcinol and triamcinolone acetonide acetate were 103.18-515.90 μmg · mL-1 (r=1.0000) and 1.336-6.680 μg · mL-1 (r=0.999 4), respectively. The average recoveries were 99.3% (RSD=0.62%) and 99.1% (RSD=0.73%), respectively.CONCLUSION:
The proposed method is simple, rapid and accurate. It has been applied in the assay of samples with satisfactory results. Copyright 2012 by the Chinese Pharmaceutical Association.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2012
Type:
Article
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