Proliferation inhibition and apoptosis induction of cucurmosin on human pancreatic cancer CFPAC-1 cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the mechanism that cucurmosin (CUS) induces the apoptosis of human pancreatic cancer cell line CFPAC-1. METHODS: The inhibition effect of cucurmosin on CFPAC-1 cell was detected by MTT assay. The apoptosis was observed by transmission electron microscope. The cell cycle and apoptosis rate were analyzed by flow cytometry. The expressions of caspase-3 and bcl-2 protein were determined by Westernblot. RESULTS: After CFPAC-1 cells were treated with cucurmosin of 0.03125, 0.0625,0.125,0.25,0.5, 1 and 2 μmol · L-1 for 24,48 and 72 h, the proliferation of CFPAC-1 cells was inhibited in a time- and dose- dependent manner(P<0.05). After CFPAC-1 cells were treated with 1 μmol · L-1 cucurmosin for 72 h, typical apoptosis changes were observed under transmission electron microscope. Compared with control group, more cells were arrested at G0/G1 phases (P<0.05) and fewer cells were at S phases(P<0.05). CUS decreased the speed of cell-cycle progression from G0/G1 phase into S phase. After CFPAC-1 cells were treated without(control) or with cucurmosin of 0.062 5,0.25 and 1 μmol · L-1 for 72 h, the apoptosis rates of CFPAC-1 cells were (0.33±0.37)%, (19.26±1.49)%,(37.13±2.07)% and (55.64±2.91)%, respectively. The expression of caspase-3 was elevated, whereas the expression of Bcl-2 was lessened gradually. CONCLUSION: Cucurmosin induces the apoptosis of pancreatic cancer CFPAC-1 cells through up-regulating the expression of caspase-3 and down-regulating the expression of bcl-2.