Separation and purification of naphthaquinone components from rhizoma of Arnebia euchroma(Royle) johnst by medium and low pressure preparative chromatography / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 923-926, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-860724
ABSTRACT
OBJECTIVE:
To establish a new chromatography technique to, separate and purify naphthaquinone components from rhizoma of Arnebia euchroma(Royle).METHODS:
Medium and low pressure preparative chromatography and Flash column were used to separate and purify the constituents, and their structures were elucidated by physicochemical properties, IR, MS, 1H-NMR and C-NMR.RESULTS:
Six monocases were obtained, which were deoxyshikonin, shikonin, isobutylshikonin, acetylshikonin, β-ace-toxyisovalerylshikonin, and β, β-dimethylacrylshikonin. The purities of the 6 compounds analyzed by HPLC were 99.62%, 99.02%, 99.5%, 99.31%, 99.3% and 99.2%, respectively.CONCLUSION:
A medium and low pressure preparative chromatography method has been established for the first time which can accurately and rapidly separate monocases from herbs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2012
Type:
Article
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