Determination of Copen in rat plasma by high performance liquid chromatography with UV detection / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a sensitive and selective HPLC-UV method for the determination of Copen in rat plasma. METHODS: After a simple sample preparation procedure by one-step protein precipitation with acetonitrile, Copen and the internal standard paeonol were separated on a Symmetry(4.6 mm×150 mm, 5 μm) column with detection wavelength at 318 nm. The mobile phase consisted of acetonitrile-water containing 0.1% phosphoric acid (5050). The flow rate was 1.0 mL·min at room temperature. RESULTS: The calibration curve was demonstrated to be linear over the concentration range of 0.101-20.2 μg·mL-1. The method had a lowest limit of quantification of 0.101 μg·mL-1. The extraction recoveries were in the range of 69.19% -77.19%. The intra-day and inter-day RSDs were below 5.20%. CONCLUSION: The method was proved to be convenient, sensitive, rapid and suitable for pharmacokinetic study. Copyright 2012 by the Chinese Pharmaceutical Association.