Inhibitory effect and mechanisms of betulinc acid on esophageal squamous carcinoma cell / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the inhibitory effect of betulinc acid(BA) on esophageal squamous cell carcinoma (ESCC) KYSE170 cells and the mechanisms of the inhibitory effect. METHODS: Different concentrations of BA(0, 5, 10, 20, 40, 60, 80 and 100 μg·mL-1) were used to detect their effects and the inhibitory rate on the cells for 24 to 72 h. The clone formation test was used to detect the long term inhibitory effects of different BA concentration(0, 5, 20 and 40 μg·mL-1) on KYSE170 cells. Different concentrations of BA(10, 60 and 100 (μg·mL-1) for 24 and 48 h were used to detect the apoptosis rate of cells by flow cytometry. Different concentrations of BA(0, 10 and 40 μg·mL-1) for 24 h were used to detect the cell cycle of cells by flow cytometry. RESULTS: BA inhibited the growth of KYSE170 cells in a dose- and time-depentent manner. IC50 at 24, 48 and 72 h were (56.81±2.56), (39.73±2.77) and (29.28±3.05) μg·mL-1, respectively. The clone formation plating efficiencies were (89.56±5.00)%, (61.00±2.03)%, (31.33±3.51)% and (15.33±2.33)% when the drug concentrations were 0, 5, 20 and 40 μg·mL-1, respectively. With the increase of BA concentration and the prolong of BA incubation time the apoptosis rate increased significantly (P<0.05 or 0.01). After 0, 1 and 10 μg·mL-1 BA added for 24 h, the rate of Gl phase cells decreased, while the rate of S phase cells increased(P<0.01). CONCLUSION: BA can inhibit the growth of KYSE170 cell by inducing cell apoptosis and blocking cells to stay in S phase. Copyright 2012 by the Chinese Pharmaceutical Association.