Targeting gene delivery to prostatic cancer with mAb-conjugated polyamidoamine dendrimers / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 418-422, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-860784
ABSTRACT
OBJECTIVE:
Aim to construct a novel polymer gene delivery system based on a new kind of dendrimer-polyamidoamine (PAMAM). With the modification of polyethylene glycol (PEG) and YPSMA-1 monoclonal antibody (mAb), PAMAM-PEG-mAb was successfully synthesized as a novel gene vector targeting to the prostatic cancer.METHODS:
NMR was used to characterize PAMAM-PEG-mAb. The cellular uptake and prostatic cancer (PCa) distribution experiments were employed to explore its biological characteristics and PCa cell (PC3 and LNCaP) targeting ability.RESULTS:
NMR results demonstrated the successful synthesis of PAMAM-PEG-mAb. The cellular uptake of vectors was concentration-dependent. The gene expression in vitro indicated that the modification of mAb could increase the gene expression efficiency and PCa targeting ability of PAMAM vectors to LNCaP ( PSMA overexpressing prostate cancer cells).CONCLUSION:
PAMAM-PEG-mAb is a potential gene delivery vector targeting to PCa. Copyright 2012 by the Chinese Pharmaceutical Association.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2012
Type:
Article
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