Preparation and antioxidation activity of selened Lycium barbarum polysaccharides sulfate and its inhibitory effect on Hela cell growth in vitro / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare selened Lycium barbarum polysaccharides sulfates (Se-LBPS) and to investigate its antioxidation activity and inhibitory effect on Hela cell growth. METHODS: Se-LBPS was prepared through reaction of laboratory-made sulfated Lycium barbarum polysaccharides with sodium selenite using acedic acid as the catalyst, and it was characterized by means of Fourie transform infrared spectroscopy. The antioxidation activity was investigated by spectrophotometry, and the inhibitory action of Se-LBPS on the growth of human cervical carcinoma Hela cell in vitro was examined using MTT method. RESULTS: The eliminating ratios of hydroxyl radical and superoxide radical for Se-LBPS achieved 60.51% and 47.9%, respectively. The growth inhibiton ratio of human cervical carcinoma Hela cell by Se-LBPS was better than LBPS and selened Lycium barbarum polysaccharides (Se-LBP). The inhibition ratio was positively correlated with Se-LBPS concentration. An inhibition ratio of 59.67% was achieved with 200 μg · mL-1 Se-LBPS. CONCLUSION: Se-LBPS has obvious antioxidation activity which is stonger than selenium sweet potatoleaf polysaccarides, and marked inhibitory effects on human cervical carcinoma Hela cell growth stonger than folic acid. Copyright 2012 by the Chinese Pharmaceutical Association.