Effect of budesonide on the expression of urotensin-II and its mRNA in asthma airway remodeling rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the effects of budesonide(BUD) on the expression of urotensin-II (UII) and its mRNA in asthma airway remodeling rats. METHODS: Twenty-four Sprague-Dawley (SD) rats were randomly divided into three groups the control group, asthma group and BUD treated group. In the experiment, the models of asthma were established by sensitization and challenge with ovalbumin (OVA). The total brochial wall thickness (Wat) and the airway smooth musle thickness (Wam) were measured by image analysis system. The levels of UII in BALF and serum were measured by enzyme linked immunosorbent assay (ELISA). The protein and mRNA expression of UII were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR), respectively. RESULTS: Wat and Warn of of asthma group were significantly higher than those of the control group (P < 0.01, respectively). Compared with asthma group, those were all markedly decreased in BUD group (P < 0.01, respectively). The levels of UII in BALF and serum of asthma group were markedly higher than those in control group and BUD group (P < 0.01, respectively). Immunohistochemistry and RT-PCR showed that the protein and mRNA expression of UII in asthma group were significantly higher than those of control group (P < 0.01, respectively). While those of BUD group were significantly lower than those of asthma group(P < 0.01, respectively). There were a significantly positive correlation between Wat and the protein and mRNA expression of UII. At the same time, there were a significantly positive correlation between Warn and the protein and mRNA expression of UII. CONCLUSION: The beneficial effect of glucocorticoid (BUD) on asthma airway remodeling may be at least in part due to their direct inhibitory effect on UII. Copyright 2012 by the Chinese Pharmaceutical Association.