A comparative study of antithrombotic activities of fucoidan with different molecular weight and its mechanism / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the antithrombotic activity of fucoidan with different molecular weight from Laminaria japonica, and to explore its mechanism by observing antiplatelet activities and fibrinolysis. METHODS: FeCl3-induced arterial thrombosis in rats were used to examine the antithrombotic activity of four fucoidan fractions with different molecular weight; the TXB2, 6-keto-PGF1α, PLG, t-PA and PAI-1 in plasma were determined by ELISA to evaluate the inhibitory effects of the drugs on platelet aggregation and fibrinolytic system. RESULTS: Four fucoidan fractions with different molecular weight could significantly decreased the weight of the thrombus, and Middle molecular weight (MMW) fucoidan fractions exhibited better antithrombotic activity than low molecular weight fucoidan (LMW). Administration of LMW fucoidan significantly decreased the TXB2 content and promoted 6-keto-PGF1α content. Administration of MMW fucoidan only decreased the TXB2 content and didn't change 6-keto-PGF1α content. In four fucoidan fractions group, the activity of t-PA were increased, the activity of PAI-1 were decreased, the ratio of t-PA/PAI-1 was increased, and the activity of PLG had no change. CONCLUSION: Four fucoidan fractions with different molecular weight have strong anti-thrombosis effect, the action mechanism may have correlation with the increase in the activities of anti-platelet aggregation and fibrinolysis system. Copyright 2012 by the Chinese Pharmaceutical Association.