Effects of echinacoside on striatal extracellular levels of hydroxyl radical in cerebral ischemia rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To examine the effects of echinacoside (ECH) on striatal extracellular levels of hydroxyl radical in cerebral ischemia rats and its possible mechanism of anti-cerebral ischemia. METHODS: Rats were divided into control, model, ECH high and low dose and CXQ groups randomly. Every rat was administered drugs or vehicle through intraperitoneal injection, one time a day for 7 consecutive days. At day 3, focal ischemia was generated by permanent middle cerebral artery occlusion (MCAO). Then the striatal extracellular fluids were gained by brain microdialysis. High performance liquid chromatography with electrochemical detection was used to measure the striatal extracellular levels of hydroxyl radicals. RESULTS: The levels of 2, 3-DHBA, 2, 5-DHBA increased rapidly. Compared with the model group, administration of ECH of high and low dose (30, 15 mg · kg-1 · d-1) and CXQ (30 mg · kg-1 · d-1) successfully prevented the elevation of 2, 3-DHBA and 2, 5-DHBA. CONCLUSION: ECH can reduce striatal extracellular levels of hydroxyl radical, which may be one of its mechanisms of anti-cerebral ischemia. Copyright 2012 by the Chinese Pharmaceutical Association.