Preparation and characterization of capsosome as a novel sandwich-type drug carrier / 中国药学杂志

ABSTRACT

OBJECTIVE: To fabricate a novel sandwich-type drug carrier called "capsosome" using natural polyelectrolytes such as chitosan and alginate, then characterize the structure, drug loading and release profile. METHODS: Chitosan and alginate and DMPG (Dimyristoyl Phosphatidylglycerole) liposomes were coated on the CaCO3 microparticles using layer-by-layer assembly technique. Upon removal of the CaCO3 template core, stable capsosomes, containing one layer of intact liposomes as cargo, were obtained; As a model drug, DOX with different concentrations was used to evaluate the drug loading ability of capsosome, the cumulative release a-mount was determined at different time points. RESULTS: Confocal laser scanning microscope images and transmission electron microscope images indicated that liposomes were embeded in the microcapsule shell successfully, drug loading amount of the obtained capsosomes increased with the DOX incubation concentration increasing (100-1000 μg · mL-1), the highest loading amount could reach to 30.47 × 10-3 ng/each capsosome, the cumulative release amount was about 46% in 48 h. CONCLUSION: The capsosome had favourable drug loading and release ability, it deserved to be studied as a novel drug carrier. Copyright 2012 by the Chinese Pharmaceutical Association.