Simultaneous determination of the contents of astragaloside IV, danshensu, protocatechualdehyde, ginsenosides Rg1 and Rb1 in Qishenyiqi dropping pills by LC-MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a LC-MS method for simultaneous determination of 5 compounds (astragaloside IV, danshensu, protocatechualdehyde, ginsenosides Rg1 and Rb1) in Qishenyiqi Dropping Pills. METHODS: The chromatographic separation was carried out at 30°C on a Wonda Sil C18 column (4.6 mm × 150 mm, 5 μm) eluted in a gradient program. The mobile phase consisted of water containing 10 mmol · L-1 ammonium formate and 0.2% formic acid-acrtonitrile containing 0.2% formic acid. The mass spectra were obtained by Agilent 6410 triple quad mass spectrometer with electrospray ionization source in negative ion mode. RESULTS: The linear ranges for astragaloside IV, danshensu, protocatechualdehyde, ginsenosides Rg1 and Rb1 were 10.72-536.0, 89.10-4 455, 1.337-66.87, 22.60-1 130 and 23.55-1 177 ng · mL-1, respectively. The average recoveries ranged between 98%-102%. CONCLUSION: The established method is convenient, sensitive and accurate. It can be used for the determination of the contents of astragaloside IV, danshensu, protocatechualdehyde, ginsenosides Rg1 and Rb1 in Qishenyiqi Dropping Pills for quality control.