Preparation of MRI contrast of dual-targeted cell adhesion molecules with microparticles of iron oxide and in vitro experiment / 中国医学影像技术
Chinese Journal of Medical Imaging Technology
;
(12): 805-811, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-861323
ABSTRACT
Objective:
To evaluate the specificity and efficacy of combination of dual-targeted MRI contrast agent cell adhesion molecule (CAM)-microparticles of iron oxide (MPIO) and endothelial cells.Methods:
The single-targeted contrast agent intercellular adhesion molecule (ICAM)-MPIO, vascular cell adhesion molecule (VCAM)-MPIO and dual-targeted contrast agent CAM-MPIO were synthesized, then Prussian blue staining, immunofluorescence and MR scanning were applied to estimate the specificity and efficacy of combination of contrast agents and tumor necrosis factor-α (TNF-α) activated endothelial cells.Results:
Prussian blue staining showed much stronger blue granules surrounding the stimulated cells in CAM-MPIO group than in ICAM-MPIO group and VCAM-MPIO group. Immunofluorescence essay demonstrated that the yellow fluorescence area per cell of CAM-MPIO group was (2.00±0.31) times and (2.46±0.45) times higher than that of ICAM-MPIO group and VCAM-MPIO group. In vitro MRI showed the signal intensity of T2WI and T2 value decreased with the increase concentration of targeted contrast agents, especially in CAM-MPIO group.Conclusion:
Dual-targeted probe CAM-MPIO may be more valuable than single-targeted probe ICAM-MPIO and VCAM-MPIO for imaging diagnosis of early radiation-induced brain injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Medical Imaging Technology
Year:
2019
Type:
Article
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