Ultrasound guided intra-amniotic instillation of pulmonary surfactant combined with ambroxol for prevention of lung injury in preterm rabbits / 中国医学影像技术

ABSTRACT

Objective: To investigate the value of ultrasound guided intra-amniotic instillation of pulmonary surfactant (PS) combined with prenatal ambroxol hydrochloride (AH) for prevention of lung injury in preterm rabbits. Methods Totally 20 pregnant New Zealand rabbits were randomly divided into 4 groups (each n=5), i.e. ultrasound guided intra-amniotic instillation of PS before cesarean section (PS group), prenatal AH (AH group), ultrasound guided intra-amniotic instillation of PS combined with prenatal AH (PS+AH group) and control group. On the 27th day of pregnancy, rabbit fetuses were removed through cesarean section 1 hour after injection. The survival time of the preterm rabbits, the concentration of interleukin-6 (IL-6) and dipalmitoyl phosphatidylcholine (DPPC) in bronchoalveolar lavage fluid, the expression level of pulmonary surfactant-associated proteins-A (SP-A) in lung tissue were detected. And the lung tissue sections were taken to observe the degree of lung injury. Results: Compared with control group, the survival time of the preterm rabbits of PS group, AH group and PS+AH group prolonged, the concentration of IL-6 decreased, the concentration of DPPC and SP-A increased, and the pathological score of lung injury decreased (all P<0.05). There were significant differences between PS+AH group and PS, AH group in those indexes except for survival time (all P<0.05). Conclusion: Ultrasound guided intra-amniotic instillation of PS combined with prenatal AH plays a role in the prevention of lung injury in preterm rabbits.