Intravoxel incoherent motion DWI in assessment of hypoxic status of tumor microenvironment of C6 glioma modle in SD rats / 中国医学影像技术

ABSTRACT

Objective: To explore the feasibility of intravoxel incoherent motion DWI (IVIM-DWI) in assessment of hypoxic status of tumor microenvironment of C6 glioma in SD rat models. Methods C6 glioma models were established in 48 SD rats. IVIM-DWI scanning were performed on 16 rats on the 14th day, 21st day and 28th day after modeling, respectively, and parameters of ADC, slow diffusion coefficient (D), fast diffusion coefficient (D*) and fractional perfusion-related volume (f) were obtained. The rats were scarified after each scanning, and the tumors were removed for hypoxia-inducible factor (HIF-1α) immunohistochemical staining and scoring. Differences of IVIM-DWI parameters and HIF-1α score at different time points were compared, and correlation between parameters of IVIM-DWI and HIF-1α score were analyzed. Results: There were statistical differences of IVIM-DWI parameters and HIF-1α score on the 14th day, 21st day and 28th day after modeling (all P<0.01). On the 21st day after modeling, HIF-1α score was negatively correlated with D, D* and f (r=-0.73, -0.58, -0.67, all P<0.05), while on the 28th day after modeling, HIF-1α score were negatively correlated with D and f (r=-0.60, -0.65, both P<0.05). Conclusion: IVIM-DWI can be used to assess hypoxic status of tumor microenvironment of C6 glioma SD rat models.