Therapeutic Effect of Hydrogen Sulfide on Esophageal Remodeling in Animal Model of Achalasia / 胃肠病学

ABSTRACT

Background: Achalasia is an esophageal motility disorder with unclear etiology. It is characterized by impaired relaxation of the lower esophageal sphincter (LES) caused by irreversible damage to the myenteric plexus of esophagus. Remodeling of lower esophageal smooth muscle has been observed in patients with achalasia. Aims: To investigate the effect of hydrogen sulfide on expression of esophageal remodeling biomarker in animal model of achalasia. Methods: The animal model of achalasia was established by administering benzyldimethyltetradecylammonium chloride (BAC) into the LES in BALB/c mice. Fifteen model mice (treatment group) were administered with sodium hydrosulfide, an exogenous hydrogen sulfide donor, intraperitoneally. The results of esophageal manometry, as well as the expressions of two esophageal remodeling biomarker (actin and elastin, determined by immunohistochemistry) were compared between model mice with or without hydrogen sulfide treatment and the blank control mice. Results: The mouse model of achalasia was established successfully. Compared with the blank control group, the LES pressure and expressions of actin and elastin were significantly increased in model group and treatment group (P<0.05), and the increase was less in treatment group than in model group (P<0.05). Conclusions: Remodeling of lower esophagus is existed in animal model of achalasia. Hydrogen sulfide can inhibit the esophageal remodeling, which provides a theoretical basis for the treatment of achalasia with hydrogen sulfide.