Screening and Regulatory Network Analysis of Core MiRNAs in Gastric Cancer Based on Bioinformatics / 胃肠病学
Chinese Journal of Gastroenterology
;
(12): 603-609, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-861765
ABSTRACT
Background:
MicroRNAs (miRNAs) are negative regulators of gene expression in various eukaryotes and play roles in RNA silencing and post-transcriptional regulation. Gene expression is affected by miRNAs dysregulation in almost all types of malignancies.Aims:
To explore the core miRNAs regulatory network of gastric cancer, and to provide a theoretical basis for the analysis of molecular mechanism of miRNAs in the development of gastric cancer.Methods:
MiRNAs and mRNA expression profile microarray were used to screen the differentially expressed miRNAs and mRNA. MiRWalk 2.0 was used to predict miRNAs-mRNA interactions, cross-matching with genes were selected by expression profile microarray to define the core differentially expressed miRNAs; and miRNAs-mRNA regulatory network was constructed. GO analysis and KEGG analysis were performed to analyze the targeted gene.Results:
Twenty-one up-regulated miRNAs and 36 down-regulated miRNAs in gastric cancer were screened by expression profile microarray. After cross-matching, 1 042 low-expressed genes and 711 high-expressed genes were found, and 10 core miRNAs-mRNA regulatory networks were finally defined. The differentially expressed genes regulated by the core miRNAs were mainly involved in tumor-related pathways, and the high-expressed genes were mainly enriched in 9 signaling pathways such as ECM-receptor interaction, while the low-expressed genes were mainly enriched in 5 signaling pathways such as neuroactive ligand-receptor interaction. GO analysis showed that the up-regulated genes involved 315 functions and the down-regulated genes involved 88 functions, of which extracellular matrix organization was the most relevant.Conclusions:
MiRNAs-mRNA regulatory network analysis based on bioinformatics provides a new perspective for gastric cancer research, which helps to systematically elucidate the molecular mechanism of miRNAs in the development of gastric cancer. It could provide a theoretical basis for the screening of biomarkers and the precise target selection for drug treatment of gastric cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Journal of Gastroenterology
Year:
2019
Type:
Article
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