Effect of Down-regulating NEDD9 Expression on Biological Behavior of Colon Cancer / 胃肠病学

ABSTRACT

Background: NEDD9 plays an important role in cell migration, chemotaxis, apoptosis, and cell cycle. NEDD9 siRNA can effectively reduce the gene expression, promote apoptosis and block tumor cell migration and invasion at different levels. Aims: To investigate the expression of NEDD9 in colon cancer tissue, and the effect of NEDD9 siRNA on proliferation, apoptosis, migration and invasion of colon cancer Caco-2 cells. Methods: Colon cancer tissue and corresponding paracancerous tissue from Jan. 2017 to Jan. 2018 at Yancheng District People's Hospital of Luohe were collected. Colon cancer Caco-2 cells were conventionally cultured, and were transfected by LipofectamineTM 2000. Caco-2 cells were divided into control group, negative control group and siRNA interference group. The mRNA expression of NEDD9 in colon cancer tissue and Caco-2 cells was detected by qRT-PCR. The proliferation of Caco-2 cells was detected by CCK-8 method. The apoptosis of Caco-2 cells was detected by flow cytometry. Caco-2 cell migration and invasion were detected by Transwell assay. The protein expressions of NEDD9, MMP-9, Bcl-2, Bax, TIMP1 were determined by Western blotting. Results: The mRNA expression of NEDD9 in colon cancer tissue was significantly higher than that in paracancerous tissue (P<0.05). Compared with control group, the mRNA expression of NEDD9 in the siRNA interference group was significantly decreased (P<0.05), cell proliferation, migration and invasion were significantly inhibited (P<0.05), cell apoptosis was significantly increased (P<0.05), and the protein expressions of NEDD9, MMP-9 and Bcl-2 were significantly decreased (P<0.05), the protein expressions of Bax and TIMP1 were significantly increased (P<0.05). Conclusions: NEDD9 gene can regulate the proliferation, apoptosis, migration and invasion of colon cancer Caco-2 cells through apoptosis-related genes Bcl-2 and Bax and invasion-related genes MMP-9 and TIMP1.