Molecular Mechanism of Helicobacter pylori-associated Diseases Based on MiRNA-mRNA Regulatory Network / 胃肠病学

ABSTRACT

Background: Helicobacter pylori (Hp) infection is the leading cause of chronic gastritis, duodenal ulcer, and gastric cancer, and is associated with a variety of extragastrointestinal diseases. However, the molecular pathogenic mechanism of Hp and its interaction with the host are still unclear. Aims: To provide clues for understanding the interaction between Hp and host and the Hp-associated diseases through the establishment of a miRNA-mRNA regulatory network and bioinformatics analysis. Methods: Datasets from Gene Expression Omnibus (GEO) were used to determine the differentially expressed miRNAs and mRNAs in patients with Hp infection. Intersection of differentially expressed mRNAs and targeted mRNAs of the differentially expressed miRNAs was identified to form a miRNA-mRNA regulatory network. Gene ontology (GO)/KEGG enrichment analyses were performed, a protein-protein interaction network was constructed and the possible Hp-associated diseases were analyzed. Results: Gene set enrichment analysis revealed that the mostly enriched gene set was chemokine receptors bind chemokines. Using disease-related analysis of 28 core node genes in protein-protein interaction network, Hp infection was found to be associated with cancers and diseases of digestive, nervous, cardiovascular, respiratory and immune systems. Conclusions: Comprehensive analysis provides a method for predicting pathogen-related human diseases based on miRNA-mRNA regulatory network and points out that Hp infection might increase the risk of many extragastrointestinal diseases.