Preparation of heat-sensitive lipid nanoparticles and effect in identification of ablation boundary / 中国介入影像与治疗学

ABSTRACT

Objective: To prepare heat-sensitive lipid nanoparticles (HLNP) capsuling liquid perfluorocarbon, and to observe the phase-shift of HLNP under heating and the effect in microwave ablation in vitro. Methods: HLNP were prepared, and the real-time phase-shift effect of HLNP caused by heating plate was observed using the light microscope, and HLNP-included void hydrogel phantom heated by microwave on ultrasound imaging was observed. Then the echo changes of ultrasound and macroscopic changes of bovine liver injected with HLNP around the microwave ablation range were explored. Results: The mean particle size of the prepared HLNP was (507.90±101.70)nm. Phase-shift of HLNP began when the temperature of heating plate reached 45℃, and a large number of microbubbles were gradually generated under 50℃. The ultrasonic echo of HLNP significantly enhanced after microwave heating. In vitro bovine liver experiment showed that the bovine liver injected with HLNP changed from red to white after microwave ablation and ultrasonic echo significantly enhanced around the microwave ablation gasification zone. Conclusion: Liquid-gas phase transition of the prepared HLNP happened under heating and microwave thermal ablation, indicating that the prepared HLNP may be used as a kind of thermal sensitizer for identifying microwave ablation boundary and real-time monitoring effect for microwave ablation.