Preparation of folate-targeted phase-transition nanoparticles carrying bismuth sulfide and application in CT/ultrasound imaging in vitro / 中国介入影像与治疗学

ABSTRACT

Objective To prepare folate-targeted phase-transition nanoparticles carrying bismuth sulfide (FBS-PFH-NPs), in order to use for targeting performance and CT/ultrasound (US) imaging in vitro. Methods Rotary evaporator and probe-type sonication methods were used to prepare FBS-PFH-NPs. Basic characteristics of FBS-PFH-NPs were detected using several analytical methods. The targeting performance of FBS-PFH-NPs was verified through incubation with cervical cancer Hela cells in vitro. The echo intensity and temperature variation of FBS-PFH-NPs were observed after irradiating with HIFU using different acoustic powers (60, 90, 120, 150 and 180 W), respectively. The effects of FBS-PFH-NPs with different Bi2S3 concentrations (1.0, 2.0, 3.0, 4.0 and 5.0 mg/ml) for enhancing CT/US imaging were investigated. Results FBS-PFH-NPs were prepared with diameter (458.50±69.22)nm, which showed regular spherical morphology and uniform size under microscope. Bi2S3 nanoparticles randomly distributed in the lipid shell, and the concentration of Bi2S3 was 1.0 mg/ml. A mass of FBS-PFH-NPs was bond to Hela cells. The phase-transition of FBS-PFH-NPs occurred after irradiation with HIFU. The echo intensities and temperature of FBS-PFH-NPs gradually enhanced with increased HIFU powers (F=110.09, 440.69, both P<0.01). The echo intensities and CT value of FBS-PFH-NPs gradually enhanced with increased Bi2S3 concentrations (F=146.14, 16.74, both P<0.01). Conclusion FBS-PFH-NPs can not only target to Hela cell specifically, but also be applied in CT/US dual-modal imaging in vitro.