Transcriptome of congenital talipes equinovarus based on RNA sequencing / 国际儿科学杂志

ABSTRACT

Objective:To sequence the whole transcriptomics of congenital talipes equinovarus(CTEV)by RNA sequencing(RNA-Seq)and to compare the differential expression of mRNA, long non-coding RNA(lncRNA), circular RNA(circRNA)and microRNA(miRNA)between CTEV and normal children.Methods:RNA was extracted from plasma samples of 3 CTEV children(experimental group)and 3 normal children(control group). After RNA-Seq, the differentially expressed genes(DEG)and transcripts of CTEV and normal children were screened out.Gene ontology analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analysis were carried out for the DEG.Results:The gene expression profile was successfully constructed.There were 181 DGE in the experimental group and control group, including 58 up-regulated genes and 123 down-regulated genes.A total of 23 lncRNA, 12 up-regulated and 11 down-regulated, were differentially expressed in the present study.There were 23 miRNA differentially expressed, including 11 up-regulated and 12 down-regulated miRNA.No differential expression of circRNA was found in the present study.The expression of secreted phosphoprotein 1(SPP1)and ribosomal protein S27(RPS27)was significantly different in the experimental group and the control group with the fold change being 82.24 and 0.01, respectively.Conclusion:DGE in the plasma samples of CTEV and normal children were analysed by full transcriptome sequencing.SPP1 and RPS27 may be the candidate genes of CTEV.