YAP ameliorates TNF-α induced intestinal epithelial barrier damage / 国际儿科学杂志

ABSTRACT

Objective:To investigate the protective role of YAP in TNF-α induced intestinal epithelial barrier injury.Methods:Caco-2 cells was cultured 14d to establish the intestinal mucosal barrier model, and then they were transfected with YAP plasmid and control plasmid vector, and 24h later they were exposed to TNF-α, and 48h later cell viability was quantified by CCK-8 assays.Cell cycle was determined by flow cytometric analysis.Cell migration was monitored by Transwell.Results:Compared to the normal control group, after exposed to TNF-α, the growth rate of Caco-2 cells was significantly decreased, and the ratio of G0/G1 phase cells was significantly higher, while the S phase and G2/M phase proportion decreased, and markedly reduced cells proliferation.The over expression of YAP significantly ameliorated the decrease of the growth rate induced by TNF-α, and promoted the cell cycle in G0/G1 phase to S phase and G2/M phase transition.Transwell results showed that the number of cell migration in control group was(172.4±13.1), after adding TNF-α stimulation decreased significantly(80.4±9.3), while the number of cell migration induced by transfection of YAP was(124.2±9.2). The difference was statistically significant( P<0.01). Conclusion:YAP inhibits intestinal epithelial barrier injury induced by TNF-α through regulating intestinal epithelial cell survival and migration.