SEPT9, IRF4, BCAT1 and IKZF1 gene methylation in the clinical diagnosis of colorectal cancer / 国际生物医学工程杂志

ABSTRACT

Objective:To investigate the separate detection and combined detection of plasmin 9 (SEPT9), interferon regulatory factor 4 (IRF4), branched-chain amino acid transaminase 1 (BCAT1) and Ikaros family zinc finger protein 1 (IKZF1) gene methylation in blood samplesin clinical diagnosis of colorectal cancer.Methods:105 patients with colorectal cancer who received surgical treatment in the Department of Colorectal Oncology, Tianjin Medical University Cancer Institute and Hospital from February 2015 to May 2018 were selected as the case group. At the same time, 105 healthy subjects with similar age range and sex ratio were selected as the control group.The blood samples and clinical information of the study subjects were collected,and methylation-specific PCR was used to detect the methyl groups of SEPT9, IRF4, BCAT1 and IKZF1 genes in blood samples. The correlation between single gene and combined gene methylation level and clinicopathological characteristics of colorectal cancer was analyzed.Results:The positive rates of SEPT9, IRF4, BCAT1 and IKZF1 single gene methylation in blood samples of the case group respectively were 67% (70/105), 44% (46/105), 45% (47/105) and 46% (48/105), which were higher than those of the control group (0), and the differences were statistically significant (all P<0.001). The positive rate of the combined detection of the above four gene methylation in blood samples of the case group was 76%, which was higher than that of the single gene methylation detection. The single gene methylation of SEPT9, IRF4, BCAT1, IKZF1 and the combined methylation of the 4 genes in blood samples were all related to the clinical stage, maximum tumor diameter, progression-free survival (PFS) and overall survival (all P<0.05). Conclusions:The combined detection of blood SEPT9, IRF4, BCAT1 and IKZF1 gene methylation can significantly improve the positive rate of detection results. This paper provides a theoretical basis for the early diagnosis of colorectal cancer.