Effect of low-level laser therapy on cytokines in gingival crevicular fluid of patients with severe periodontitis / 国际生物医学工程杂志

ABSTRACT

Objective:To study the effect of low-level laser therapy (LLLT) on cytokines in gingival crevicular fluid of patients with severe periodontitis, and to analyze the effect of LLLT on periodontal inflammation control and bone healing.Methods:A total of 43 teeth from 18 patients with severe periodontitis were selected. There were no differences in age, gender, and periodontal status of all patients enrolled. 20 teeth in the control group received basic periodontal treatment, and 23 teeth in the experimental group were received combination of basic periodontal treatment and LLLT. During the LLLT treatment, the gingival pocket was irradiated by low-level laser for 15 s on days 1, 2, and 7. One and four weeks after treatment, the gingival crevicular fluid the patients in the control group and the experimental group was collected, and the interleukin-1β (IL-1β), transforming growth factor-β (TGF), tumor necrosis factor-α (TNF-α), metal matrix protease-8 (MMP-8), soluble intercellular adhesion molecule-1 (sICAM-1) and periosteal protein levels were determined by enzyme-linked immunosorbent assay (ELISA).Results:After treatments, the levels of IL-1β, TNF-α, MMP-8 and sICAM-1 in the control group and the experimental group decreased, and the levels of TGF-β and periosteal protein increased. The basic periodontal treatment combined with LLLT had better therapeutic effect. One week after treatments, the differences in the levels of IL-1β and TNF-α between the two groups were statistically significant (all P<0.05). Four weeks after treatments, the differences in the levels of IL-1β, TNF-α, TGF-β, MMP-8 and sICAM-1 between the two groups were statistically significant (all P<0.05). Conclusions:LLLT treatment can effectively reduce the levels of IL-1β, TNF-α and MMP-8 in the gingival crevicular fluid of patients with severe periodontitis in a short period of time. LLLT has a certain effect on the recovery of periodontal tissue, including controlling periodontal inflammation, reducing the expression of sICAM-1 in gingival crevicular fluid, alleviating the symptoms of gum bleeding, promoting the secretion of TGF-β, increasing the level of periosteal protein, and inhibiting the destruction of periodontal tissue.