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Effect of long-chain non-coding RNA AL117378.1 on the proliferation and invasion of bladder cancer cells / 国际外科学杂志
International Journal of Surgery ; (12): 13-17,封3, 2020.
Article in Chinese | WPRIM | ID: wpr-863262
ABSTRACT
Objective To investigate the effect of long-chain non-coding RNA AL117378.1 on the proliferation and invasion of bladder cancer cells.Methods From August 2016 to December 2017,the tumor tissues and adjacent tissues of 14 patients with bladder cancer treated by Huangshi Central Hospital of Edong Healthcare Group were selected.Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of AL117378.1 in 14 pairs of bladder cancer tissues and corresponding adjacent tissues,bladder cancer cell lines (5637,J82,T24,BIU-87) and corresponding human bladder normal epithelial cells (SV-HUC-1).The negative control plasmid (control group) and the plasmid carrying the AL117378.1 (experimental group) were transfected into the bladder cancer cell line with the lowest expression level.qRT-PCR was used to detect the expression levels of ALl 17378.1 and non-receptor protein tyrosine phosphatase 9 (PTPN9)mRNA in the transfected cells.The expression levels of PTPN9,E-cadherin,o-SMA,CDK4 and Cyclin A2 protein were detected by Western blotting.MTT assay and Transwell invasion assay were used to detect the cell proliferative capacity and invasive ability of the two groups.Measurement data were expressed as mean ± standard deviation(Mean ± SD),and comparison between groups used independent sample t test.Results The expression of AL117378.1 in bladder cancer tissues was lower than that in adjacent tissues (P < 0.01).The expression of AL117378.1 in bladder cancer cell lines was lower than that in human bladder normal epithelial cells (P < 0.01),and the expression level of AL117378.1 was the lowest in J82 cells (P < 0.01).The expression levels of AL117378.1 in the control and experimental groups were 1.02 ± 0.11 and 7.96 ± 1.06,respectively,and the difference was statistically significant (t =6.51,P <0.01).The expression of PTPN9 mRNA was 1.01 ± 0.08 and 4.99 ± 0.50,respectively.the difference was statistically significant (t =7.84,P <0.01).Western blotting results showed that the expression of PTPN9 and E-cadherin increased,and the expression of α-SMA,CDK4 and Cyclin A2 decreased.MTT experiments showed that the proliferation of cells transfected with AL117378.1 was significantly decreased (P < 0.05).Transwell invasion experiments showed that the number of invasive cells in the control group and the experimental group were 97.96 ± 13.71 and 38.02 ±7.51,respectively,and the difference was statistically significant (t =3.84,P < 0.01).Conclusions The expression of AL117378.1 was significantly decreased in bladder cancer tissues and cell lines (5637,J82,T24,BIU-87).ALl 17378.1 can significantly inhibit the proliferation and invasion of bladder cancer cells by positively regulating the expression of PTPN9 gene.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Surgery Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Surgery Year: 2020 Type: Article