Bioinformatics analysis of TUBB3 expression and its regulatory mechanisms in non-small cell lung cancer / 国际肿瘤学杂志

ABSTRACT

Objective:To predict the expression and molecular regulatory mechanisms of βⅢ-tubulin (TUBB3) in non-small cell lung cancer (NSCLC) using bioinformatics methods.Methods:The GEO profile, TransmiR, miRWalk and ConSite database were employed in the present study. The upstream transcription factor, co-expression gene and the interactive miRNA of TUBB3 were studied to determine the molecular regulatory mechanisms and possible role of TUBB3 in NSCLC.Results:The promoter region of TUBB3 could be recognized by transcription factors associated with NSCLC including Snail and n-MYC and so on. At the post-transcription level, the TUBB3 could be regulated by miRNAs including miR-200 family, miR-342-3p, miR-410 and so on. These miRNAs were associated with proliferation and invasion of NSCLC. TUBB3 was co-expressed with HDGF, GMPS, MRPL9, PMAIP1 and SLBP, and they were co-regulated by the transcription factor Snail. SLBP, PMAIP1 and transcription factor Snail were related to cell cycle and apoptosis.Conclusion:The expression of TUBB3 can be regulated at multiple levels in NSCLC. It may take part in cell cycle and apoptosis regulation in NSCLC, and further influence proliferation and invasion of cancer cells.