Identification of the chemical constituents of Fangfeng-Tongsheng Pills by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 670-678, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-863659
ABSTRACT
Objective:
To identify the chemical constituents of Fangfeng-Tongsheng Pills by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Orbitrap HRMS).Methods:
Take Agilent InfinityLab Poroshell 120 C18 column (150 mm×3.0 mm, 2.7 μm) and make water containing 0.1% formic acid and acetonitrile as gradient eluents. Data was collected with positive and negative ion modes using full scan and two-stage threshold-triggered mass modes (Full MS/dd-MS 2) and HESI source. The main chemical constituents of Fangfeng-Tongsheng Pills were identified according to the exact molecular mass, the cleavage fragments of MS 2, the retention time of reference substances, mzVault 2.0 MS database and literature review.Results:
A total of 82 chemical constituents including 33 flavonoids, 13 chromones, 8 triterpenoid saponins, 6 coumarin, 5 iridoids, 4 phthalides, 4 terpenoids, 3 phenolic acids, 3 lignans, 2 anthraquinone and 1 alkaloids, were detected and identified.Conclusions:
UPLC-Q-Orbitrap HRMS could identify the main chemical constituents of Fangfeng-Tongsheng Pills rapidly and accurately, which may provide a theoretical basis for further studies on the mechnism and quality control of this formula.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
International Journal of Traditional Chinese Medicine
Year:
2020
Type:
Article
Similar
MEDLINE
...
LILACS
LIS