Study on the mechanism of Coptidis rhizoma in treating gastritis based on network pharmacology / 国际中医中药杂志

ABSTRACT

Objective:To investigate the mechanism of Coptidis rhizoma in the treatment of gastritis based on network pharmacology. Methods:The main components and targets of Coptidis rhizoma were screened by TCMSP and TCMID database. GeneCards were used to select the target genes related to gastritis from the human gene database, and the target genes related to gastritis were screened by Genemap in the OMIM database. We used R language VennDiagram package to crosse the gene targets of drugs and diseases, and screen the target of the main components of Coptidis rhizoma in the treatment of gastritis, and to use Cytoscape software to map the gene regulatory network of galangal in the treatment of gastritis, and to use the String database to construct the gene-protein of Coptidis rhizoma. The interaction visualization network map for protein- protein interaction (PPI) were screened out the core genes, and the Bioconductor in the R language was used to analyze the GO and KEGG pathways for the selected gene targets. Results:Fourteen main active compounds of Coptidis rhizoma were screened, and 71 gene targets may be involved in the treatment of gastritis. The results of GO and KEGG pathway analysis indicated that berberine mainly involves cytokine receptor binding, regulation of cytokine activity, and biological processes such as binding to heme, by regulating AGE-RAGE, IL-17, TNF, NF-kappa B and HIF-1. The signaling pathway acts to treat gastritis. Conclusions:This study constructed the role of berberine in the treatment of gastritis with multi-active component, multi-gene, multi-target pathway through network pharmacology. It comprehensively predicted the gene target and signaling pathway of Coptidis rhizoma in the treatment of gastritis, in order to further study the treatment of berberine. The mechanism of action of gastritis provides a reference basis.