Effect of curcumin on the growth and invasion of thyroid carcinoma K1 cells / 国际中医中药杂志

ABSTRACT

Objective:To investigate the effect and possible mechanism of curcumin on the growth and invasion of thyroid carcinoma papillary K1 cells.Methods:K1 cells were randomly divided into control group, solvent control group, and curcumin group with 10, 30 and 50 μmol/L. The control group was only added with fresh medium, the solvent control group was added with dimethyl sulfoxide medium, and the 10, 30, 50 μmol/L curcumin groups were added with 10, 30 and 50 μmol/L curcumin respectively. The effects of curcumin on the growth of thyroid cancer papillary carcinoma K1 cells were observed by MTT assay. PI and Hochest3342 double staining were used to detect cell death. Transwell chamber was used to verify the effect on cell invasion. Western blot was used to observe the effects of curcumin on expression of Vimentin and E-cadherin.Results:Compared to the control group and solvent control group, the cell survival rate significantly decreased and the cell death rate of 10, 30, 50 μmol/L curcumin groups significantly increased ( P<0.05). The cell invasion rate (80.12% ± 3.43%, 65.59% ± 4.11%, 30.32% ± 4.67% vs. 100.00% ± 2.81%, 98.82% ± 2.18%) of 10, 30, 50 μmol/L curcumin groups significantly decreased ( P<0.05). The expression of E-cadherin (0.41 ± 0.12, 0.63 ± 0.14, 0.70 ± 0.13 vs. 0.34 ± 0.10, 0.35 ± 0.11) of 10, 30, 50 μmol/L curcumin groups significantly increased ( P<0.05), and the expression of Vimentin (0.70 ± 0.11, 0.22 ± 0.10 vs. 0.87 ± 0.12, 0.87 ± 0.13) of 10, 30, 50 μmol/L curcumin groups significantly decreased ( P<0.05). Conclusions:Curcumin can inhibit the proliferation and invasion of papillary thyroid carcinoma K1 cells and accelerate cell death.