Extraction and separation of apigenin-7-O-glucoside and TLC identification in Folium malloti apeltae / 国际中医中药杂志

ABSTRACT

Objective:To establish the extraction and separation method of flavone from the Folium malloti apeltae and TLC identification method. Methods:The Folium malloti apeltae was extracted by 95% ethyl alcohol, and the ethanol recovery by rotary distillation. The extracted Folium malloti apeltae was mixed well with silicone. It was eluted with petroleum ether and extracted with ethyl acetate. Then, the concentrated ethyl acetate fraction was recovered. The ethyl acetate fraction was separated and purified on a polyamide column with gradient elution of water and methanol. The TLC identification was carried out with daposthride as the reference substance, the leaves as the reference material, the silica gel G plate as the adsorbent, and chloroform methanol formic acid (1030.3) as the developing agent. Results:Apigenin-7-O-glucoside obtained from Folium malloti apeltae. TLC identification method of Folium malloti apeltae was established with detectable spots and satisfactory separating result. Conclusions:This method to identify Folium malloti apeltae is accurate and rapid, which can be used for the quality control of Folium malloti apeltae.