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The protective effect of oleanolic acid on diabetic myocardium of rats and its mechanism / 中华急诊医学杂志
Chinese Journal of Emergency Medicine ; (12): 1191-1195, 2020.
Article in Chinese | WPRIM | ID: wpr-863847
ABSTRACT

objective:

To investigate the protective effect of oleanolic acid (OA) on the heart of diabetic cardiomyopathy (DCM) rats and its mechanism.

Methods:

Fifty healthy 4-week-old male SD rats were randomly (random number) divided into the normal control group (CON), diabetes mellitus group (DM), diabetes mellitus + oleanolic acid group (DM+OA), diabetes mellitus + chloroquine group (DM+CQ) and diabetes mellitus + oleanolic acid + chloroquine group (DM+OA+CQ), with 10 rats in each group. The diabetic animal model was established by feeding high-fat and high-sugar food (35.5% wt/ wt), intra-abdominal injection of streptozotocin (STZ) 50 mg/kg 4 weeks later, and feeding high-fat and high-sugar food for another 16 weeks. OA was orally administered at 80 mg/(kg·d) for 4 weeks, and CQ was intraperitoneal injected at 10 mg/kg per day for 4 weeks. The rats were anesthetized after echocardiographic examination at the end of the 20th week. Echocardiography was used to detect the heart function of SD rats, and the autophagy related proteins LAMP2, p-ULK1, p-p70s6k, p-raptor, LC3 and p62 were semi-quantitatively analyzed by Western blot. Changes in cardiac function and expression of autophagy related proteins in cardiac myocytes of rats were observed. One-way analysis of variance was used for comparison among groups of normally distributed measurement data, SNK- q test was used for pairwise comparison between two groups.

Results:

Compared with the CON group, the end diastolic wall thickness, left ventricular end diastolic volume, and end left ventricular systolic volume [(7.58±0.25) mm, (376.6±13.6) μL, and (132.6±10.8) μL] of the DM group were lower than those of the CON group [(8.37±0.11) mm, (458.3±16.4) μL, and (166.6±12.8) μL] (all P<0.05). Compared with the DM group, the end diastolic wall thickness, left ventricular end diastolic volume, and left ventricular end systolic volume [(8.63± 0.14) mm, (473.6±18.6) μL, and (174.6±12.7) μL] of the DM+OA group were higher than those of the DM group (all P<0.05). The anterior wall thickness, left ventricular end-diastolic volume, and left ventricular end-systolic volume [(7.97± 0.17) mm, (393.6±15.6) μL, and (147.6±10.2) μL] of the DM+OA+CQ group were lower than those of the DM+OA group (all P<0.05). The expression of autophagy-related protein p-ULK1 was increased and the expression of p-p70s6k and p-raptor was decreased in the DM group (all P<0.05). Compared with the DM group, cardiac dysfunction was reduced in the OA group, accompanied by decreased p62 expression and increased LC3Ⅱ/Ⅰ expression (both P<0.05). Compared with the DM+CQ group, p62 expression was decreased and LC3 Ⅱ/Ⅰ expression was increased in the DM+OA+CQ group (both P<0.05).

Conclusions:

OA has a protective effect on the myocardium of diabetic rats, which may be related to enhanced autophagy.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Emergency Medicine Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Emergency Medicine Year: 2020 Type: Article