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miR-483-5p promote adrenocortical cancer proliferation and invasion via down-regulation of CDK15 / 中华内分泌外科杂志
Chinese Journal of Endocrine Surgery ; (6): 155-160, 2020.
Article in Chinese | WPRIM | ID: wpr-863889
ABSTRACT

Objective:

To investigate the role of miR-483-5p in adrenocortical cancer (ACC) and its possible mechanism.

Methods:

Quantitative real-time polymerase chain reaction was conducted to estimate the expression of miR-483-5p and CDK15 in ACC tissues and cell lines. The effects of miR-483-5p on proliferation were determined in vitro using CCK-8 proliferation assays, the changes of invasion of ACC cells were examined by Transwell. The molecular mechanism underlying the relevance between miR-483-5p and CDK15 was confirmed by luciferase assay and rescue assays.

Results:

We found a relatively higher miR-483-5p (2.36±1.02 vs 1.09±0.43) and lower CDK15 (0.57±0.26 vs 1.06±0.32) expression in ACC specimens and cell lines. CDK15 was verified as a direct target of miR-483-5p by luciferase assay. over-expression of miR-483-5p promoted proliferation (24 h 0.26±0.03 vs 0.23±0.04, 48 h 0.56±0.05 vs 0.41±0.03, 72 h 0.73±0.04 vs 0.59±0.03) and invasion (95.78±4.66 vs 23.89±2.52) by down-regulating CDK15 expression.

Conclusion:

miR-483-5p plays a tumorigenesis role in ACC progression by down-regulating CDK15 expression, which may lead to a novel insight to the potential biomarker and novel therapeutic strategies for ACC.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Endocrine Surgery Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Endocrine Surgery Year: 2020 Type: Article