Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats
Journal of Neurogastroenterology and Motility
;
: 171-178, 2013.
Article
in English
| WPRIM
| ID: wpr-86423
ABSTRACT
BACKGROUND/AIMS:
Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the 14C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method.METHODS:
After ingestion of a meal containing 3 mCi 99mTc and 2 microCi 14C-octanoate, 23 male Wistar rats were placed on a gamma camera in a airflow container. Scintigraphic images were taken at regular intervals. The amount of 14CO2 in a regularly replaced hyamine hydroxide solution, capturing CO2 in the outflow air, was counted using liquid scintillation spectrometry. 99mTc gastric retention curves and 14CO2-excretion curves were fitted to their respective data. Three rats underwent the same procedures after administration of atropine.RESULTS:
Overall Tr10% (time at which 10% of the original amount of 99mTc remained in the stomach) was 355 +/- 64 minutes; Te90% (time at which 90% of total amount of 14CO2 was excreted) was 325 +/- 106 minutes. Their correlation coefficient was 0.71, R-square 0.50 and P < 0.005. Tr1/2 (50% of original amount of 99mTc remained) was 124 +/- 28 minutes; Te1/2 (50% of total amount of 14CO2 excreted) 114 +/- 32 minutes. Their correlation coefficient was 0.83 with R-square of 0.69 and P < 0.00005. In 12 immobilized animals correlation was even better correlation coefficient 0.84; R-square 0.71 and P < 0.001 (Tr10% was 388 +/- 117 minutes; Te90% 532 +/- 219 minutes; Tr1/2 of 165 +/- 54 minutes; Te1/2 of 175 +/- 67 minutes). Atropine significantly lengthened all emptying times 904 +/- 307 and 1461 +/- 684 minutes for Tr10% and Te90%, respectively; and 432 +/- 117 minutes for Tr1/2 and 473 +/- 190 minutes for Te1/2.CONCLUSIONS:
We adapted and validated the 14C-octanoate gastric emptying breath test for application in rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retention, Psychology
/
Atropine
/
Spectrum Analysis
/
Benzethonium
/
Breath Tests
/
Caprylates
/
Gamma Cameras
/
Rats, Wistar
/
Eating
/
Meals
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Journal of Neurogastroenterology and Motility
Year:
2013
Type:
Article
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