Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
Biomolecules & Therapeutics
;
: 442-448, 2015.
Article
in English
| WPRIM
| ID: wpr-86474
ABSTRACT
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1beta (IL-1beta)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1beta-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Caseins
/
Gene Expression
/
Blotting, Western
/
Chondrocytes
/
Thrombospondins
/
Collagen Type II
/
Interleukin-1beta
/
Knee
/
Models, Theoretical
Limits:
Animals
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2015
Type:
Article
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