Protective effect of neurokinin 1 receptor antagonist on interleukin-13 induced oxidative stress injury of bronchial epithelial cells / 中国小儿急救医学

ABSTRACT

Objective:To investigate whether WIN 62, 577 which is an antagonist of neurokinin 1 receptor(NK-1R) has protective effects on interleukin(IL)-13-induced bronchial epithelial cell oxidative stress injury.Methods:Human bronchial epithelial cells (16HBE) were cultured and divided into 4 groups: control group, IL-13 group, IL-13+ SP group[IL-13+ sensory neuropeptide substance P(SP)] and IL-13+ WIN 62, 577 group.16HBE cells were treated with IL-13 (25 ng/ml) for 48 hours.After 48 hours, the IL-13 + SP group was treated with sensory neuropeptide SP(10 nmol/L) for 1 hour, and the IL-13+ WIN 62, 577 group was treated with WIN 62, 577 (10 nmol/L) for 1 hour, respectively.The proliferation ability, reactive oxygen species (ROS)level, malondialdehyde (MDA)content and total superoxide dismutase (SOD) activity in each group were detected by corresponding kits.Results:Compared with the control group, cell proliferation ability reduced ( P<0.001), ROS level increased ( P=0.001), MDA content increased( P<0.001), and SOD activity reduced ( P<0.001) in the IL-13 group.Compared with the IL-13 group, the cell proliferation ability reduced ( P=0.016), the ROS level increased ( P=0.031), the MDA content increased( P<0.001), and the SOD activity decreased ( P=0.011)after sensory neuropeptide SP stimulation.However, the intervention of WIN 62, 577 could inhibit the decrease of cell proliferation ( P=0.018), the increase of ROS level ( P=0.018), the increase of MDA content ( P<0.001), and the decrease of SOD activity ( P=0.001) in human bronchial epithelial cells induced by IL-13. Conclusion:Sensory neuropeptide SP could aggravate oxidative stress induced by IL-13 in human bronchial epithelial cells, while the sensory neuropeptide SP receptor NK-1R antagonist WIN 62, 577 could alleviate oxidative stress induced by IL-13 in human bronchial epithelial cells.