Adiponectin Upregulates Filaggrin Expression via SIRT1-Mediated Signaling in Human Normal Keratinocytes
Annals of Dermatology
;
: 407-413, 2017.
Article
in English
| WPRIM
| ID: wpr-86521
ABSTRACT
BACKGROUND:
Filaggrin (FLG) is the major component of the epidermal granular layer and binds to and condenses the keratin cytoskeleton. FLG thus contributes to cell compaction and serves as a natural moisturizing factor by promoting unfolding and degradation into hygroscopic amino acids. Loss or downregulation of FLG has been shown to result in a weak stratum corneum, which causes water loss and increases the possibility of skin barrier-related seizure. Adiponectin (Acrp30) contributes to the functional recovery of somatic cells, including human normal epidermal keratinocytes (NHEKs).OBJECTIVE:
To investigate the effect of Acrp30 in FLG expression and identifying its signal transduction mechanism.METHODS:
Normal human keratinocytes were treated with Acrp30 and the levels of FLG were examined. Silent mating type information regulation 2 homolog (SIRT)-targeting siRNA and aryl hydrocarbon receptor nuclear translocator (ARNT)-targeting siRNA were used to identify the role of various signal transduction pathway components.RESULTS:
Acrp30 upregulated SIRT1 and ARNT expression in NHEKs, resulting in increased FLG expression. Treatment with both SIRT1-targeting siRNA and ARNT-targeting siRNA blocked Acrp30 stimulation and silenced FLG expression.CONCLUSION:
Adiponectin upregulates FLG expression through a SIRT1-mediated pathway. Our results suggest that Acrp30 is a promising agent for skin barrier permeability improvement.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Seizures
/
Skin
/
Cytoskeleton
/
Water
/
Signal Transduction
/
Down-Regulation
/
Keratinocytes
/
RNA, Small Interfering
/
Adiponectin
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2017
Type:
Article
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