Association between rs5896 polymorphism of prothrombin gene and renal calculi in elderly patients / 中国基层医药

ABSTRACT

Objective:To investigate the relationship between rs5896 polymorphism of prothrombin gene and renal calculi in the elderly patients.Methods:Ninety elderly patients with kidney stones admitted to Hangzhou Hospital of Zhejiang Medical and Health Group from January 2014 to December 2016 were selected as observation group, and another 80 healthy people during the same period were selected as control group.The prothrombin gene rs5896 polymorphism was detected by RT-PCR, and the differences between the two groups were compared.Results:The T frequency of rs5896 allele in the observation group (64.44%) was higher than that in the control group (46.25%) (χ 2=5.687), and the TT genotype (54.44%) was higher than that in the control group (38.75%) (χ 2=4.187, P<0.05). TT genotype (77.78%) at rs5896 locus was more than single stone (38.89%), and CC genotype (16.67%) was less than single stone (37.04%) (χ 2=13.171, 4.363, all P<0.05). The serum uric acid level of TT type[(434.21±78.13)μmol/L] was higher than that of CC+ CT type[(245.73±54.20)μmol/L] ( t=13.038, P<0.05). The serum creatinine, calcium, phosphorus and potassium levels of different genotypes had not statistically significant differents (all P>0.05). The urine level of uric acid in TT-type [(415.32±56.63)μmol/L] was higher than that in CC+ CT type [(267.31±67.18)μmol/L] ( t=11.343, P<0.05), but there were no statistically significant differences in the changes of calcium, phosphorus and potassium in different genotypes of urine (all P>0.05). Conclusion:The T gene frequency of prothrombin gene rs5896 is higher and TT genotype frequency is higher in elderly patients with renal calculi.It is suggested that T gene may be a susceptible gene and that this site may lead to renal calculi through uric acid metabolic disorder, which is worthy of clinical reference.