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Protective effect of TFB on serum induced injury of HUVECs in henoch-schonlein purpura children patients / 中国医师杂志
Journal of Chinese Physician ; (12): 525-528,534, 2020.
Article in Chinese | WPRIM | ID: wpr-867279
ABSTRACT

Objective:

To investigate the protective effect and mechanism of total flavonoids of bidens bipinnata L (TFB) on vascular endothelial cell injury in children with henoch-schonlein purpura (HSP).

Methods:

Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and intervened with normal and HSP children's serum. Then the HUVECs were divided into blank group, normal group, HSP group and TFB group. Levels of interleukin-8 (IL-8) and tumor necrosis factor α (TNF-α) in the supernatant were detected by enzyme-linked immunosorbent assay ( ELISA). The mRNA and protein expression of nuclear factor kappa B (NF-κB) and Fractalkine were detected by reverse polymerase chain reaction (RT-PCR) and Western blot in HUVECs.

Results:

ELISA results showed that the expression of IL-8 and TNF-α in HSP group were significantly higher than that in normal control group and blank group ( P<0.05), and the level of IL-8 and TNF-α in the TFB group was significantly lower than that in the HSP group ( P<0.05); the results of RT-PCR and Western blot showed that the expression levels of NF-κB, Fractalkine mRNA and protein in HSP group were significantly higher than those in normal group and blank group ( P<0.05). The expression levels of NF-κB, Fractalkine mRNA and protein in the TFB group were significantly lower than those in the HSP group ( P<0.05).

Conclusions:

TFB can inhibit the expression of NF-κB in children with Henoch-Schonlein purpura, reduce the secretion of IL-8 and TNF-α in HUVECs, and then relieve the occurrence of vascular injury.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chinese Physician Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chinese Physician Year: 2020 Type: Article