Effect of hydrogen molecule on the formation of pelvic and abdominal adhesions in dogs and its mechanism / 中国医师杂志

ABSTRACT

Objective:To study the effects of hydrogen molecule on the formation of pelvic and abdominal adhesions in dogs, and to explore its possible mechanism.Methods:The Beagle pelvic and abdominal adhesion model was constructed. 15 Beagle dogs were randomly divided into 3 groups model control group, hydrogen treatment group and blank control group. The Phillips adhesion score was used to evalute the morphological adhesion degree. The morphology of intestinal, uterus and ovarian tissues was evaluated by hematoxylin-eosin (HE) staining. The inflammation biomarkers and oxygen free radical index were detected before modeling and on the 1st, 4th, 7th and 14th day after modeling. C-reactive protein (CRP), tumor necrosis factors-α (TNF-α) and interleukin(IL)-6 content in serum were detected by enzyme-linked immunosorbent assay (ELISA) kit; hydroxylamine method was used to detect the serum superoxide dismutase (SOD); thiobarbituric acid (TBA) colorimetric method was used to measure malondialdehyde concentration.Results:The Phillips adhesion score of the model control group was significantly higher than that of the blank control group ( P<0.05). The Phillips adhesion score of the hydrogen treatment group was significantly lower than that of the model control group ( P<0.05). In the hydrogen treatment group, the thickening of serous layer on the surface of intestine, uterus and ovary and the degree of inflammatory infiltration were lower than those in the model control group.The inflammatory indexes (leukocyte count, CRP, IL-6, TNF-α) and malondialdehyde level in the model control group were higher than those in the blank control group on the first day after modeling, and the superoxide dismutase (SOD) level was lower than that in the blank control group (all P<0.05); the inflammatory indexes and malondialdehyde level in the hy-drogen treatment group were lower than those in the model control group on the first day after modeling ( P<0.05), and the SOD level was higher than that in the model control group ( P<0.05). Conclusions:Hydrogen molecule can effectively inhibit the formation of pelvic adhesion in Beagle dogs, and its possible mechanism is to neutralize oxygen free radicals and reduce inflammatory reaction.