The expression and clinical significance of MST4, TRAF6 and NF-κB in premature ruptured of membranes with intrauterine infection / 中国医师杂志

ABSTRACT

Objective:To investigate the expression of serine/threonine protein kinase 4 (MST4), tumor necrosis factor receptor-related factor 6 (TRAF6) and nuclear factor-kappa B (NF-κB) in patients with premature rupture of membranes (PROM) complicated with intrauterine infection and their clinical value in the diagnosis of early intrauterine infection.Methods:30 normal pregnant women delivered in our hospital from September 2017 to September 2018 were selected as the control group and 90 PROM pregnant women as the experimental group. The fetal membranes were examined by hematoxylin-eosin (HE) staining. The expressions of TRAF6, MST4 and NF-κB in maternal peripheral blood, umbilical cord blood and amniotic fluid were detected by enzyme linked immunosorbent assay (ELISA) and receiver operating characteristic (ROC) curve was used to analyse the diagnostic value of the three indicators.Results:There were 54 cases of intrauterine infection in the PROM group and 36 cases of no intrauterine infection in the PROM group. ELISA results showed that the expression of TRAF6, MST4 and NF-κB in PROM with intrauterine infection group was higher than those in control group and PROM without intrauterine infection group ( P<0.05). The expressions of TRAF6, MST4 and NF-κB in maternal blood, umbilical cord blood and amniotic fluid were positively correlated. ROC curve analysis showed that NF-κB, MST4 and TRAF6 had the highest value in predicting intrauterine infection in maternal blood, umbilical cord blood and amniotic fluid, respectively. Conclusions:TRAF6, MST4 and NF-κB may be involved in the occurrence and development of PROM complicated with intrauterine infection and provide evidence for the early diagnosis of intrauterine infection.