Expression and significance of EGFL7 in malignant meningioma cells and tissues / 中国医师杂志

ABSTRACT

Objective:To investigate the expression and significance of epidermal growth factor-like domain 7 (EGFL7) in malignant meningioma.Methods:Human malignant meningeal cell lines (IOMM-LEE, CH157-MN) and normal meningeal cells (HMC-1400) were cultured in vitro. The expressions of EGFL7 mRNA and protein in cells were detected by real time fluorescent quantitative polymerase chain reaction (qRT-PCR), reverse polymerase chain reaction (RT-PCR), and Western blot. The expression levels of EGFL7 mRNA and protein in 25 cases of malignant meningioma, the corresponding normal arachnoid tissue around the tumor and the invasive skull tissue were detected. Results:The results of qRT-PCR, RT-PCR and Western blot showed that the expression levels of EGFL7 mRNA and protein in human malignant meningeal cell lines (IOMM-LEE, CH157-MN) were higher than those in normal meningeal cells (HMC-1400) ( P<0.05). The expression levels of EGFL7 mRNA and protein in malignant meningiomas were higher than those in normal arachnoid tissues around the tumors ( P<0.05); the expression levels of EGFL7 mRNA and protein in invasive cranial tissues, primary malignant meningiomas and normal arachnoid tissues around the tumors decreased in turn ( P<0.05). Conclusions:EGFL7 is highly expressed in malignant meningioma tissues and cell lines, and its high expression may be associated with malignant meningioma invasion.