Levels of serum let-7 and miR-26b in patients with diabetes mellitus and their relationships with insulin resistance / 中国医师杂志

ABSTRACT

Objective:To investigate the expressions of let-7 and microRNA-26b (miR-26b) in serum of patients with type 2 diabetes mellitus (T2DM) and their relationships with insulin resistance (IR).Methods:60 newly diagnosed patients with T2DM from March 2018 to March 2019 were selected as the T2DM group, and 70 healthy people in the same period were selected as the control group. The clinical data of the two groups were collected and the expression levels of let-7 and miR-26b in serum were measured by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), the expression levels of let-7 and miR-26b in serum of patients with T2DM and their correlations with other clinical indicators were observed, and the receiver operating characteristic (ROC) curve was used to analyze the predictive values of let-7 and miR-26b levels in serum for IR in patients with T2DM.Results:Total cholesterol (TC), triglyceride (TG), glycosylated hemoglobin (HbA1c), fasting blood glucose (FPG), fasting insulin (FINS) and homeostasis model assessment of insulin resistance (HOMA-IR) in the T2DM group were significantly higher than those in the control group ( P<0.05), while insulin sensitivity index (ISI) and homeostasis model assessment of β cell function (HOMA-β) were significantly lower than those in the control group ( P<0.05). The serum levels of let-7 and miR-26b in patients in T2DM group were significantly lower than those in the control group ( P<0.05). The serum levels of let-7 and miR-26b in patients in T2DM group were negatively correlated with TC, TG, HbA1c, FPG, FINS and HOMA-IR ( P<0.05), and positively correlated with ISI and HOMA-β ( P<0.05). ISI, HOMA-IR and the serum levels of let-7 and miR-26b were independent risk factors for IR in patients with T2DM ( P<0.05). The area under curve (AUC) of serum let-7 expression predicting IR in patients with T2DM was 0.858, and the best cut-off value was 0.73, with sensitivity and specificity 77.50% and 80.00% respectively. The AUC of miR-26b in serum predicted the occurrence of IR in patients with T2DM was 0.908, and the best cut-off value was 0.60, with sensitivity and specificity 80.00% and 92.50%. Conclusions:The expression levels of let-7 and miR-26b in serum of patients with T2DM are significantly decreased, both of them may participate in the occurrence and development of IR, and have certain predictive value for the occurrence of IR in patients with T2DM, while miR-26b is more effective.