Correlation between the deletion of phosphatase and tensin homolog gene on chromosome 10, syncytin-1 and the damage of maternal endothelium and trophoblast in preeclampsia placenta / 中国综合临床

ABSTRACT

Objective:To study the correlation between phosphatase and tensin homolog deleted on chromosome ten(PTEN), Syncytin-1 in preeclamptic placenta and maternal endothelial injury, trophoblast injury.Methods:From February 2015 to December 2017, 46 patients diagnosed as preeclampsia and delivered in Xiaolan Hospital Affiliated to Southern Medical University and 80 pregnant women with normal delivery in the same period were selected.Before delivery, maternal peripheral blood was collected and the content of endothelial injury markers was determined; after delivery, the placenta was retained and the expression of PTEN, Syncytin-1, apoptosis gene and the contents of oxidative stress markers were measured.Results:The mRNA expression of PTEN in placental tissue of preeclampsia was significantly higher than that of normal placental tissue( t=32.797, P<0.000), and the mRNA expression of Syncytin-1 was significantly lower than that of normal placental tissue( t=28.506, P<0.001); the content of ET-1 and sFlt-1 in the blood circulation of the maternal body of preeclampsia was significantly higher than thsoe of normal pregnant women( t=39.884, 40.243; all P<0.000) and positively correlated with the mRNA expression of PTEN in placenta(correlation coefficien t=0.674, 0.596; P=0.004, 0.007), negatively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficien t=-0.615, -0.637; P<0.009, 0.008); the content of NO, PGI2, VEGF and PLGF were significantly lower than thse of normal pregnant women( t=30.944, 27.404, 32.827, 26.644; all P<0.001) and negatively correlated with the mRNA expression of PTEN in placenta(correlation coefficien t=-0.648, -0.592, -0.537, -0.613; P=0.006, 0.009, 0.013, 0.007), positively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficien t=0.632, 0.572, 0.537, 0.654; P<0.010, 0.013, 0.015, 0.009); The contents of NOX4, MDA, 8-OHdG and the mRNA expression of Fas, FasL, Bax, GRP78, CHOP, Caspase-3 in placental tissue of preeclampsia was significantly higher than that of normal placental tissue( t=27.714, 27.704, 26.786, 27.964, 22.622, 25.496, 25.893, 30.837, 28.975; all P<0.001) and positively correlated with the mRNA expression of PTEN in placenta(correlation coefficien t=0.703, 0.651, 0.593, 0.628, 0.449, 0.552, 0.612, 0.569, 0.502; P<0.003, 0.007, 0.004, 0.006, 0.016, 0.010, 0.006, 0.012, 0.018), negatively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficien t=-0.615, -0.714, -0.577, -0.548, -0.512, -0.585, -0.619, -0.495, -0.662; P<0.009, 0.001, 0.013, 0.015, 0.018, 0.011, 0.009, 0.023, 0.003); The contents of PrxII, PrxIII, SOD and mRNA expression of Bcl-2 in placental tissue of preeclampsia was significantly lower than that of normal placental tissue( t=26.115, 23.502, 17.194; all P<0.000) and negatively correlated with the mRNA expression of PTEN in placenta(correlation coefficien t=-0.713, -0.657, -0.591, -0.642; P<0.001, 0.006, 0.009, 0.007), positively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficien t=0.562, 0.711, 0.682, 0.608, P<0.013, <0.001, 0.002, 0.008). Conclusion:The high expression of PTEN and the low expression of syncytin-1 in placenta of preeclampsia can aggravate the damage of endothelium and trophoblast.