Sequence analysis of VP7 gene of G9 group A rotavirus in Xiamen area / 中华传染病杂志

ABSTRACT

Objective:To understand the genetic variations of the coding gene of the main neutralizing antigen VP7 of G9 group A rotavirus in Xiamen area and the difference between VP7 gene in some domestic and broad areas. Methods:Four types G9 group A rotavirus strains were collected from feces of children with diarrhea in Xiamen Children′s Hospital. The time of collection was October 5, 2017, November 12, 2017, December 7, 2017 and January 15, 2018, respectively. The full-length sequence of type G9 group A rotavirus VP7 gene was obtained by reverse transcription polymerase chain reaction, and the homology, phylogenetic and amino acid sequence alignment were analyzed by using DNA Star, MEGA and other biological software. Results:Phylogenetic tree analysis showed that the local strain of Amoy CHINA/2018 in Xiamen area and the human/SC7/CHN/2013/G9 local strain in Chengdu City had the most closely relationship in the evolutionary tree clusters, and the homology was far from the Hu/JS2016 local strain in Jiangsu Province. The amino acid sequence analysis showed that compared with the reference strain WI61, the Xiamen local strains had variations in the amino acid primary structure, including D100N and Y144H, which were representative mutation sites in the Amoy CHINA/2018 phylogenetic tree cluster.Conclusion:The full-length genome sequence of the type G9 group A rotavirus VP7 shows that the strain is mutated in China.