Construction and transcranial sonography detection of Parkinson′s disease rat models / 中华超声影像学杂志

ABSTRACT

Objective:To observe and evaluate the imaging of the substantia nigra (SN) in the midbrain of 6-hydroxydopamine(6-OHDA)-constructed PD rat model by transcranial sonography (TCS), and to observe whether the substantia nigra hyperechogenicity (SNH) in nigral region appeared stably, for the purpose of providing a scientific method and theoretical basis for the selection and construction of laboratory animal disease model of PD.Methods:Rats were divided into vehicle group and 6-OHDA group, and received stereotactic injection. After 15 days, TCS was performed to observe both model and vehicle group whether the characteristic SNH appeared in ipsilateral substantia nigra compared with contralateral. The rats were sacrificed after detection, and the number of dopaminergic (DA) neurons and fibers in the nigrostriatal pathway of each rat were observed by immunofluorescence staining. Protein expression levels were observed by western blot. These experimental results of the 6-OHDA group and the vehicle group were compared to evaluate whether the model was successfully constructed and compared with the performance of their ultrasound images.Results:Nine rats survived PD model group and vehicle group. 6-OHDA PD rat models appeared stable SNH under the TCS examination, and the average area of SNH was (3.258±0.220)cm 2. Immunofluorescence staining showed apparently loss of TH-ir cells; Western blot showed TH protein level of ipsilateral dramatically decreased. Conclusions:The SNH consistently emerges in 6-OHDA unilateral rat PD models with relatively high survival rate and success rate.6-OHDA rat models can be used to study the unclear mechanism of SNH.