The experimental study of bone marrow mesenchymal stem cells for the treatment of chronic pancreatitis in rats / 中华普通外科杂志

ABSTRACT

Objective To investigate the effects and mechanisms of BMSCs on CP in rats.Methods 40 SD rats were divided into 4 groups with 10 in eachcontrol group,model group,treatment group,and sham treatment group.The pancreatic fibrosis and pathological score were evaluated.The expression of α-SMA,collagen type Ⅰ and Ⅲ,IL-10 in pancreatic tissue were detected by ELASA.The expression of apoptosis genes BNIP3 in pancreatic tissue was assayed by qRT-PCR.The protein of TGF-β1,Smad2,Smad3 and Smad4 in TGF-β/Smad signal pathway in pancreatic tissue was determined by Westernblot method.The culture system of PSC was divided into 2 groups with 5 in eachcontrol group and treatment group.The expression of α-SMA,collagen type Ⅰ and Ⅲ,IL-10 for PSC were detected by ELASA.The expression of apoptosis genes BNIP3 for PSC was assayed by qRT-PCR.The protein of TGF-β1,Smad2,Smad3 and Smad4 in TGF-β/Smad signal pathway for PSC was determined by Western-blot method.Results (1) The pancreatic fibrosis and pathological score in treatment group were lower than model group and sham treatment group (P ＜ 0.05).The expression of α-SMA,collagen type Ⅰ and Ⅲ in tissues were less in treatment group compared with model group and sham treatment group (P ＜ 0.05) while the level of BNIP3 and IL-10 in pancreatic tissue were higher in treatment group compared with model group and sham treatment group with significant group (P ＜ 0.05).The level of TGF-β1,Smad2,Smad3 and Smad4 were lower in treatment group compared with model group and sham treatment group (P ＜ 0.05).(2) The expression of α-SMA,collagen type Ⅰ and Ⅲ were less in treatment group compared with control group (P ＜ 0.05).The level of BNIP3 and IL-10 were higher in treatment group compared (P ＜ 0.05).The level of TGF-β1,Smad2,Smad3 and Smad4 were less in treatment group compared with control group (P ＜ 0.05).Conclusion BMSCs reduce the activation and proliferation of PSC and then lower pancreatic fibrosis degree in rats with chronic pancreatitis.